Journal: Nature communications

Article Title: Reactive oxygen species-responsive and Raman-traceable hydrogel combining photodynamic and immune therapy for postsurgical cancer treatment.

doi: 10.1038/s41467-022-32160-z

Figure Lengend Snippet: Fig. 4 | In vivo ROS-responsive characteristics of the hydrogel. a Optical and Raman images of Ce6@PPG samples, with and without LED irradiation, at different time points post implantation. b Fluorescence IVIS images depicting the in vivo retention of Ce6 fluorescence in mice with Ce6 solution (Free Ce6), AFIgG/ Ce6@PPG with (+L) or without (−L) the 640 nm LED irradiation. c Mean fluores- cence intensity (MFI) of corresponding fluorescence IVIS images in b.

Article Snippet: 4-Dimethylaminopyridine (DMAP) and dimethyl sulfoxide (DMSO)werepurchased fromSinopharmChemical Reagent Co., Ltd. Chlorin e6 (Ce6) was purchased from Frontier Scientific, Inc. aCD47 (Catalog no. BE0270) used in vivo was purchased from BioX Cell.

Techniques: In Vivo, Irradiation, Fluorescence

Journal: Journal of Nanobiotechnology

Article Title: Hemin-incorporating DNA nanozyme enabling catalytic oxygenation and GSH depletion for enhanced photodynamic therapy and synergistic tumor ferroptosis

doi: 10.1186/s12951-022-01617-0

Figure Lengend Snippet: Schematical showing the preparation of DF with AS1411 G4 motif for Ce6 loading and hemin incorporation for tumor targeting PDT/ferroptosis combinatorial therapy

Article Snippet: Hemin and Chlorin e6 (Ce6) were purchased from Frontier Scientific Co., Ltd (Utah, USA).

Techniques:

Journal: Journal of Nanobiotechnology

Article Title: Hemin-incorporating DNA nanozyme enabling catalytic oxygenation and GSH depletion for enhanced photodynamic therapy and synergistic tumor ferroptosis

doi: 10.1186/s12951-022-01617-0

Figure Lengend Snippet: A Schematic showing the design of template sequence and the preparation of DF. B The TEM micro-images of DF and CH/DF. C The SEM micro-image of CH/DF. D DLS and E surface charge measurements of DF and CH/DF. Inset: the appearance of the nanoparticles after centrifugation. F The fluorescence spectra of Ce6 before and after loading into DF. G The elemental mapping micro-image of CH/DF

Article Snippet: Hemin and Chlorin e6 (Ce6) were purchased from Frontier Scientific Co., Ltd (Utah, USA).

Techniques: Sequencing, Centrifugation, Fluorescence

Journal: Journal of Nanobiotechnology

Article Title: Hemin-incorporating DNA nanozyme enabling catalytic oxygenation and GSH depletion for enhanced photodynamic therapy and synergistic tumor ferroptosis

doi: 10.1186/s12951-022-01617-0

Figure Lengend Snippet: A Ex vivo imaging the biodistribution of CH/DF and free Ce6 at 12 h post-injection into tumor bearing mice. B The intensity quantification at different organs based on the fluorescence images in A . C Dynamic monitoring the tumor growth after various treatments. D The appearance and E weight of the tumors at day 16 post various treatments. F Tumor H&E staining images, fluorescent staining of TUNEL and caspase-3 of the mice with different treatments

Article Snippet: Hemin and Chlorin e6 (Ce6) were purchased from Frontier Scientific Co., Ltd (Utah, USA).

Techniques: Ex Vivo, Imaging, Injection, Fluorescence, Staining, TUNEL Assay

Journal: IET Nanobiotechnology

Article Title: Mn‐doped ZnS quantum dots‐chlorin e6 shows potential as a treatment for chondrosarcoma: an in vitro study

doi: 10.1049/iet-nbt.2018.5387

Figure Lengend Snippet: TGA analyses of the QDs, QD‐ED and QD‐ED‐Ce6

Article Snippet: 1‐ethyl‐3‐[3‐dimethylaminopropyl] carbodieimide hydrochloride (EDC) were purchased from Sigma–Aldrich (Munich, Germany) and Chlorin e6 (Ce6) were purchased from Medkoo (USA).

Techniques:

Journal: IET Nanobiotechnology

Article Title: Mn‐doped ZnS quantum dots‐chlorin e6 shows potential as a treatment for chondrosarcoma: an in vitro study

doi: 10.1049/iet-nbt.2018.5387

Figure Lengend Snippet: Cell viabilities for free Ce6, free QD and QD‐Ce6 complexes (0.5 µmol l−1) before and after X‐ray treatment on SW1353 cells, respectively

Article Snippet: 1‐ethyl‐3‐[3‐dimethylaminopropyl] carbodieimide hydrochloride (EDC) were purchased from Sigma–Aldrich (Munich, Germany) and Chlorin e6 (Ce6) were purchased from Medkoo (USA).

Techniques:

Journal: Biomolecules

Article Title: Analysis of Self-Assembled Low- and High-Molecular-Weight Poly-L-Lysine–Ce6 Conjugate-Based Nanoparticles

doi: 10.3390/biom14040431

Figure Lengend Snippet: Preparation of LPL and PLCe6. ( a ) Schematic diagram of the structure and chemical synthesis of LPL; ( b ) measurement of the molecular weight of LPL with MALDI-TOF; ( c ) schematic diagram of the structure and chemical synthesis of PLCe6; ( d ) confirmation of the synthesis of LPL1Ce6 and HPL10Ce6 molecules from Ce6 with UV–vis spectroscopy after purification; ( e ) RP-HPLC of Ce6, LPL1Ce6 and HPL10Ce6.

Article Snippet: Fetal bovine serum (FBS) was obtained from Gibco (Waltham, MA, USA) and chlorin e6 (Ce6) was obtained from Leap Chem (Hong Kong).

Techniques: Molecular Weight, UV-Vis Spectroscopy, Purification

Journal: Biomolecules

Article Title: Analysis of Self-Assembled Low- and High-Molecular-Weight Poly-L-Lysine–Ce6 Conjugate-Based Nanoparticles

doi: 10.3390/biom14040431

Figure Lengend Snippet: Cellular uptake and cytotoxicity effects of PLCe6 nanoparticles. ( a ) Cellular uptake of PLCe6 nanoparticles in CT26.WT cells treated for 3 h was observed by using fluorescence microscopy (red; Ce6, λEx = 540 nm, λEm = 605 nm, blue; DAPI, λEx = 375 nm, λEm = 460 nm); scale bars = 50 µm for all. ( b ) Cytotoxicity evaluation of Ce6, LPLCe6, and HPLCe6 on CT26.WT cells for 24 h with laser irradiation; ( c ) without laser irradiation ( n = 6), mean ± SD, *** p < 0.001.

Article Snippet: Fetal bovine serum (FBS) was obtained from Gibco (Waltham, MA, USA) and chlorin e6 (Ce6) was obtained from Leap Chem (Hong Kong).

Techniques: Fluorescence, Microscopy, Irradiation

Journal: mSphere

Article Title: Antimicrobial Photodynamic Therapy with Chlorin e6 Is Bactericidal against Biofilms of the Primary Human Otopathogens

doi: 10.1128/mSphere.00492-20

Figure Lengend Snippet: Bactericidal effects of Ce6-aPDT on planktonic cultures (A) and established biofilms (B) of M. catarrhalis 7169 (Mcat), S. pneumoniae EF3030 (Spn), and NTHi 86-026NP (NTJi). Total light dosage was 12 J/cm 2 (100 mW/cm 2 , 2 min) using 1 μM Ce6 for all planktonic assessments and 90 J/cm 2 (100 mW/cm 2 , 15 min) using 10 μM Ce6 for biofilm assays. Control sets were exposed to Ce6 but not light (+PS–L), light in the absence of Ce6 (–PS+L), or were not treated (–PS–L). Survival of bacteria, both planktonic and biofilm-associated, was determined by viable plate counts. All bacteria exposed to aPDT treatment (+PS+L) were significantly decreased in viability compared to that of the controls (–PS–L) with a >99.9% kill rate. The dashed lines indicate the limit of detection. The data are presented as the means ± SDs, with individual data points shown. ns, not significant; * , P = 0.0327; *** * , P < 0.0001.

Article Snippet: High-purity (>98%) lyophilized chlorin e6 trisodium salt (Ce6) (Fotolon; Apocare Pharma GmbH, Bielefeld, Germany) was handled in the dark to restrict ambient light exposure.

Techniques: Control, Bacteria

Journal: mSphere

Article Title: Antimicrobial Photodynamic Therapy with Chlorin e6 Is Bactericidal against Biofilms of the Primary Human Otopathogens

doi: 10.1128/mSphere.00492-20

Figure Lengend Snippet: Viability of biofilm-detached bacteria (hatched bars) was assessed by evaluating surviving CFU in the surrounding saline supernatant following aPDT of M. catarrhalis 7169 (Mcat), S. pneumoniae EF3030 (Spn), and NTHi 86-026NP (NTHi) biofilms (solid bars). There were no surviving bacteria (≤10 CFU) detected in the surrounding saline following Ce6-aPDT of the biofilms compared to the untreated control groups (≥10 6 CFU), indicating that bacteria that detach from the biofilms in response to aPDT are not viable. The dashed line indicates the limit of detection. The data are presented as the means ± SDs, with independent replicates shown. *** * , P < 0.0001.

Article Snippet: High-purity (>98%) lyophilized chlorin e6 trisodium salt (Ce6) (Fotolon; Apocare Pharma GmbH, Bielefeld, Germany) was handled in the dark to restrict ambient light exposure.

Techniques: Bacteria, Saline, Control

Journal: mSphere

Article Title: Antimicrobial Photodynamic Therapy with Chlorin e6 Is Bactericidal against Biofilms of the Primary Human Otopathogens

doi: 10.1128/mSphere.00492-20

Figure Lengend Snippet: Representative SEM images (×5,000 magnification) of M. catarrhalis 7169 (A), S. pneumoniae EF3030 (B), and NTHi 86-026NP (C) biofilms before (left) and after (right) Ce6-aPDT treatment.

Article Snippet: High-purity (>98%) lyophilized chlorin e6 trisodium salt (Ce6) (Fotolon; Apocare Pharma GmbH, Bielefeld, Germany) was handled in the dark to restrict ambient light exposure.

Techniques:

Journal: mSphere

Article Title: Antimicrobial Photodynamic Therapy with Chlorin e6 Is Bactericidal against Biofilms of the Primary Human Otopathogens

doi: 10.1128/mSphere.00492-20

Figure Lengend Snippet: Efficacy of two sequential treatments of Ce6-aPDT was evaluated on biofilms of M. catarrhalis 7169 (A) and S. pneumoniae EF3030 (B). NTHi 86-026NP biofilms were omitted from this analysis because the single aPDT treatment was sterilizing. Biofilms were subjected to one round of Ce6-aPDT (1× aPDT at a total light dosage of 90 J/cm 2 using 10 μM Ce6; solid bars) or immediately retreated with a second round (2× aPDT) using the exact same treatment parameters (hatched bars). Dual treatment completely eradicated all viable biofilm-associated bacteria (≤10 CFU) compared to untreated controls (≥10 5 CFU) in both organisms. The dashed lines indicate the limit of detection. The data are presented as the means ± SDs, with independent replicates shown. ns, not significant; * , P = 0.0281; *** * , P < 0.0001.

Article Snippet: High-purity (>98%) lyophilized chlorin e6 trisodium salt (Ce6) (Fotolon; Apocare Pharma GmbH, Bielefeld, Germany) was handled in the dark to restrict ambient light exposure.

Techniques: Bacteria

Journal: mSphere

Article Title: Antimicrobial Photodynamic Therapy with Chlorin e6 Is Bactericidal against Biofilms of the Primary Human Otopathogens

doi: 10.1128/mSphere.00492-20

Figure Lengend Snippet: Ce6-aPDT is effective at preventing bacterial regrowth and biofilm reestablishment 24 h following dual treatment. Biofilms of M. catarrhalis 7169 (A), S. pneumoniae EF3030 (B), and NTHi 86-026NP (C) were exposed to a single (gray bars, open symbols) or dual (white bars, closed symbols) Ce6-aPDT treatments (total light dosage of 90 J/cm 2 using 10 μM Ce6). The surviving biofilm-associated bacteria were enumerated to assess viability immediately following treatment ( t = 0, solid bars) or 24 h posttreatment (t = 24 h, lined bars). The dashed lines indicate the limit of detection. The data are presented as the means ± SDs, with independent replicates shown. ns, not significant; ** * , P = 0.0008; *** * , P < 0.0001.

Article Snippet: High-purity (>98%) lyophilized chlorin e6 trisodium salt (Ce6) (Fotolon; Apocare Pharma GmbH, Bielefeld, Germany) was handled in the dark to restrict ambient light exposure.

Techniques: Bacteria